Sample Aging Profoundly Reduces Monocyte Responses in Human Whole Blood Cultures

Pneumococcus Cultures in Whole Fresh Blood

1. It has been shown that the whole uncoagulated blood of immune animals is not as highly pneumococcidal in vitro, as has been claimed by others. 2. Cultures of pneumococci in the fresh whole blood of immune animals, as compared with cultures in the blood of susceptible animals, show a greatly prolonged latent period, and, in a general way, the relative lengths of the latent periods of the cult...

Human Blood Monocyte Subsets

Measurement of T-lymphocyte responses in whole-blood cultures using newly synthesized DNA and ATP.

The proliferative response is most frequently determined by estimating the amount of [(3)H]thymidine incorporated into newly synthesized DNA. The [(3)H]thymidine procedure requires the use of radioisotopes as well as lengthy periods of incubation (>72 h). An alternative method of assessing T-lymphocyte activation in whole-blood cultures involves the measurement of the nucleotide ATP instead of ...

Whole Blood RNA Analysis, Aging and Disease

Establishment of long-term monocyte suspension cultures from normal human peripheral blood

The long-term suspension growth of normal, immature myeloid cells from fresh human cord blood was recently reported and required cells separated on supplemented discontinuous Percoll gradients, growth in media containing hydrocortisone and vitamins D3, and gentle, continuous agitation (13). When normal adult bone marrow (six donors) or blood from Epstein-Barr virus (EBV)-seropositive donors (ni...